Nucleotide and amino acid sequences 
4.2.010
If your European patent application discloses nucleotide or amino acid sequences (unbranched sequences of four or more amino acids or unbranched sequences of ten or more nucleotides), the description must include a sequence listing in electronic format complying with WIPO Standard ST.25 or, as of 1 July 2022, WIPO Standard ST.26. You are advised to use the EPO's free BiSSAP or PatentIn software (for sequence listings in Standard ST.25 filed up until 30 June 2022) or WIPO's free "WIPO Sequence" desktop tool (for sequence listings in Standard ST.26 filed as of 1 July 2022), which simplify the creation of ST.25 txt or ST.26 xml sequence listings. The sequence listing must not be filed on paper or in PDF format. However, if it is, the sequence listing on paper or in PDF format must be identical to that in electronic format and you must submit a statement of identity. 
OJ EPO 2013, 542
OJ EPO 2011, 372
Electronic sequence listings are usually filed online, using one of the EPO's online filing tools. Uploading to EPO Form 1001 is enabled if you indicate that the application includes a sequence listing by ticking the relevant checkbox (Tab "Biology/Application details"). Further advice is obtainable from the EPO by contacting Customer Services at www.epo.org/contact.
4.2.011
The standardised presentation of such nucleotide and amino acid sequences in electronic form is mandatory. If you do not comply with the requirements even following an invitation to do so, including payment of the late furnishing fee, your European patent application will be refused (but see point 5.10.007).

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