T 1148/00 (Seed material/OY PANIMOLABORATORIO) 17-06-2002

Claim request filed with letter dated 13 Mai 2002

Formal requirements; Articles 123(2) and 84 EPC

1. A basis for the subject-matter of claims 1 to 3 is found in the application as filed in the passage bridging page 5, line 34 to page 6 line 5 as well as page 6, lines 13 to 18 and page 10, lines 29 and 30. The requirements of Article 123(2) EPC are fulfilled.

2. In the light of the description, in particular Example 5, it is clear that the term "...derived from species Lactobacillus plantarum (DSM 7388)..." in the expression "... a lactic acid bacteria preparation or a preparation produced by lactic acid bacteria derived from species Lactobacillus plantarum (DSM 7388)..." applies to the lactic acid bacteria preparation as well as to the preparation produced by lactic acid bacteria. Claims 1 to 3 fulfill the requirements of Article 84 EPC.

Novelty; Article 54 EPC

3. No documents of the state of the art on file disclose a procedure for treatment of seed material wherein the seeds are treated with Lactobacillus plantarum DSM 7388 in connection with the germination step. The subject-matter of claims 1 to 3 is novel (Article 54 EPC).

Inventive step; Article 56 EPC

4. The closest prior art is document (4) which describes a process for the conservation of germinated seeds which includes treating these seeds with lactic acid bacteria (lactobacilli or lactic streptococci) after germination has taken place (page 6, lines 1 to 11, page 11, lines 8 to 11). This treatment is said to eliminate the need for sterilisation (see, for example, page 12, lines 7 to 10) ie it prevents the multiplication of unwanted microbes.

5. Starting from the closest prior art, the problem to be solved may be defined as setting up a process for producing germinated seeds free of contamination.

6. The solution provided is to treat the seeds in connection with the germination process with a specific lactic acid bacteria: Lactobacillus plantarum DSM 7388.

7. In the Board's judgment, the person skilled in the art aware from document (4) of the inhibitory effect of lactic acid bacteria on microbes likely to develop on germinated seeds and wanting to prevent microbial development during germination would find it obvious to try and use lactic acid bacteria for their purpose; all the more so, that the state of the art does not report any deleterious effect of lactic acid bacteria on the germination process per se and that testing the properties of these bacteria in this respect (ie adding them to seeds at the onset of germination) is a matter of routine experimentation. Thus, the use of lactic bacteria in a process for producing germinated seeds, in order to inhibit microbial growth is not considered to involve inventive skills.

8. The Appellants argued that the specific Lactobacillus strain (DSM 7388) with which the claimed process is to be carried out had surprisingly good inhibitory properties which justified acknowledging inventive step.

9. The inhibitory properties of Lactobacillus plantarum DSM 7388 are described in the examples given in the patent application:

- Table 2 shows that Lactobacillus plantarum DSM 7388 (identified in the table as Lactobacillus E-76) has the same inhibitory effect as two other lactobacilli strains: Lactobacillus curvatus E-391 and Lactobacillus plantarum E-98, against all gram-negative bacteria tested including Enterobacter agglonerans, said effect being measured by the turbidometric method. Using the disk method (Table 1), 12% more inhibition of Enterobacter agglomerans is achieved with Lactobacillus plantarum DSM 7388 than with Lactobacillus plantarum E-98 (Lactobacillus curvatus not being tested). This effect being quite small and not observed by the turbidometric method, it is not considered to be meaningful.

- Figure 6 shows the results of an experiment wherein the effect of Lactobacillus plantarum DSM 7388 and Lactobacillus plantarum E-98, of suppressing retard in mash filtration is tested. As this retard is known to be caused by gram-negative bacteria (see Legend to Table 2), the experiment is an indirect measurement of the inhibitory effect of both lactobacilli on said bacteria. From studying the curves depicting the amount of filtrated mash as a function of time, it is not evident that when the seeds used to produce the mash have been treated with Lactobacillus plantarum DSM 7388 or with Lactobacillus plantarum E-98 (curves F and G), the delay in mash filtration is significantly reduced compared with the delay observed in the controls (curves A and B) which the Board takes to be representative of the time needed for mash filtration when the seeds used to produce the mash have not been treated with lactic acid bacteria. Alternatively, if the control curve is that labelled "poor", then it must be concluded that both Lactobacillus plantarum DSM 7388 and E-98 suppress this delay to about the same extent.

- The inhibitory effects of Lactobacillus plantarumDSM 7388 and E-98 and of Lactobacillus curvatus E-391 on Aspergillus niger are about identical (Table 2). DSM 7388 is better than Lactobacillus curvatus E-391 at inhibiting the growth of Fusarium moulds. Its performance, however, is only slightly better than that of Lactobacillus plantarum E-98, there being on average 5% more inhibition with the earlier strain, the data being obtained by the turbidometric method (Table 2). When compared by visual examination (Table 3), Lactobacillus plantarum DSM 7388 and E-98 are found to have the same inhibitory activity on three out of four of the tested Fusarium moulds.

Thus, the properties of Lactobacillus plantarum DSM 7388 are either identical or essentially similar to those of one out of the two other strains of lactobacilli tested. They cannot be considered surprising properties for a Lactobacillus.

10. Lactic streptococci of the genus Pediococcus have essentially the same inhibitory effect on gram-negative bacteria than Lactobacillus plantarum DSM 7388 (Tables 2 and 4). They are mostly less efficient than Lactobacillus plantarum DSM 7388 or E-98 at inhibiting moulds (Table 2) except for Pediococcus pentosaceous DSM 7389 which appears to have similar inhibiting properties as Lactobacillus plantarum DSM 7388 on the microflora of malting according to the results obtained in Example 5.

11. From the findings in points 7, 9 and 10, the Board draws the following conclusion:

- the skilled person wanting to produce germinated seeds free of contamination had the choice (obvious from document (4)) to treat the seeds to be germinated either with lactobacilli or with lactic streptococci. Applying this treatment does not in itself require inventive skill. In doing so, he/she would find out in a straightforward manner that lactobacilli are more efficient than most lactic streptococci against moulds and, thus, would obviously retain lactobacilli as the genus of choice.

- The specific lactobacillus (DSM 7388) used in theclaimed process does not exhibit surprising properties.

For these reasons, the process of claim 1 which involves treating seeds to be germinated with this lactobacillus to produce germinated seeds free of contamination is not inventive.

12. Claims 1 to 3 do not fulfill the requirements of Article 56 EPC.